ANALYSIS OF THREE-DIMENSIONAL NANOSTRUCTURES OF BIOLOGICAL MATERIALS AND OBJECTS USING SCANNING PROBE AND OPTICAL PROBE NANOTOMOGRAPHY
https://doi.org/10.56304/S2079562925010051
EDN: FAHFYZ
Abstract
This work is devoted to modern methods for studying the three-dimensional nanostructure of biomaterials and biological objects based on scanning probe nanotomography—a combination of probe microscopy and ultamicrotomy, and fluorescence microscopy. The paper presents the results of experiments on studying the three-dimensional nanostructure of composite scaffolds based on Bombyx Mori silk fibroin and extracellular matrix microparticles obtained from decellularized liver tissue. Such scaffolds have significant potential for use in regenerative medicine. It has been shown that probe nanotomography methods make it possible to effectively analyze the three-dimensional nanostructure of microinclusions and their morphological parameters that affect the biological activity of scaffolds.
About the Authors
A. E. Efimov
Academician Shumakov National Medical Research Centre of Transplantology and Artificial Organs
Russian Federation
Russian Federation
O. I. Agapova
Academician Shumakov National Medical Research Centre of Transplantology and Artificial Organs
Russian Federation
Russian Federation
I. I. Agapov
Academician Shumakov National Medical Research Centre of Transplantology and Artificial Organs
Russian Federation
Russian Federation
References
1. Zhao J., Yu X., Shentu X., et al. // Cell Tissue Res. 2024. V. 396. P. 1–18.
2. Evans J.E., Friedrich H. // MRS Bull. 2016. V. 41. P. 516–521.
3. Li X., Han Y., Liu W., et al. // Front. Phys. 2020. V. 8. P. 606217.
4. Hoffman D.P., Shtengel G., Xu C.S., et al. // Science. 2020. V. 367 (6475). P. 5357.
5. Lane R., Wolters A.H.G., Giepmans B.N.G., Hoogenboom J.P. // Front. Mol. Biosci. 2022. V. 8. P. 822232.
6. Alekseev A., Efimov A., Loos J., Matsko N., Syurik J. // Eur. Polym. J. 2014. V. 52. P. 154–165.
7. Zankel A., Wagner J., Poelt P. // Micron. 2014. V. 62. P. 66–78.
8. Mochalov K.E., Chistyakov A.A., Solovyeva D.O., et al. // Ultramicroscopy. 2017. V. 182. P. 118–123.
9. Agapova O.I., Efimov A.E., Mochalov K.E., et al. // Dokl. Biol. Sci. 2023. V. 509. P. 103–106.
10. Agapova O.I., Efimov A.E., Safonova L.A., et al. // Dokl. Biochem. Biophys. 2021. V. 500. P. 331–334.
11. Efimov A.E., Tonevitsky A.G., Dittrich M., Matsko N.B. // J. Microsc. 2007. V. 226. P. 207–217.
12. Efimov A.E., Agapov I.I., Agapova O.I., et al. // Rev. Sci. Instrum. 2017. V. 88 (2). P. 023701.
13. Alekseev A., Efimov A., Lu K., Loos J. // Adv. Mater. 2009. V. 21 (48). P. 4915–4919.
14. Mochalov K.E., Efimov A.E., Bobrovsky A., et al. // ACS Nano. 2013. V. 7 (10). P. 8953–8962.
15. Efimov A.E., Moisenovich M.M., Bogush V.G., Agapov I.I. // RSC Adv. 2014. V. 4. P. 60943–60947.
16. Safonova L., Bobrova M., Efimov A., et al. // Pharmaceutics. 2021. V. 13. P. 1561.
17. Safonova L., Bobrova M., Efimov A., et al. // Pharmaceutics. 2021. V. 13. P. 1704.
18. Efimov A.E., Agapova O.I., Safonova L.A., et al. // Express Polym. Lett. 2019. V. 13. P. 632–641.
19. Balashov V., Efimov A., Agapova O., et al. // Acta Biomater. 2018. V. 68. P. 214–222.
20. Bobrova M., Safonova L., Efimov A., et al. // Pharmaceutics. 2022. V. 14. P. 2313.
21. Ribeiro V.P., Almeida L.R., Martins A.R., et al. // J. Tissue Eng. Regen. Med. 2017. V. 11 (10). P. 2853–2863.
22. Nikkhah M., Edalat F., Manoucheri S., Khademhosseini A. // Biomaterials. 2012. V. 33. P. 5230–5346.
Review
For citations:
Efimov A.E., Agapova O.I., Agapov I.I. ANALYSIS OF THREE-DIMENSIONAL NANOSTRUCTURES OF BIOLOGICAL MATERIALS AND OBJECTS USING SCANNING PROBE AND OPTICAL PROBE NANOTOMOGRAPHY. Nuclear Physics and Engineering. 2025;16(5):698-702. (In Russ.) https://doi.org/10.56304/S2079562925010051. EDN: FAHFYZ
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